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PeptideAtlas provides a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments collected for human, mouse, yeast, and several other organisms.
(last updated: Nov 28, 2017)
Data or Model Molecule| Accession | Sample Title | Summary | Organism | Characteristics | Instrument | Treatment | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| PAe000429 | young adults (Ati: vhl-1 deletion) | young adults (Ati: vhl-1 deletion) description: young adults of a vhl-1 mutant, dissolved in 1 % Rapigest experiment type: cleavable ICAT; heavy (N2) and light (vhl-1) tag; rapigest LC-gradient: SAB_GRAD_MOD description: ? gradient programme: buffer A (10 mM KH2PO4, 25 % ACN, pH 3.0); buffer B (buffer A plus 350 mM KCl); flow 0.8 ml/min; 90 min column: 200 x 4.6 mm instrument: LTQ LC-analysis, operator: Sabine MS-analysis, operator: Sabine sample preparation: Ati, Sabine protocol: glass beads sample name: vhl-1 strain: C.elegans, vhl-1, N2 age: young adults organism, part: whole worm amount of sample: 2 mg each sample prepared on: homogenization 08.06.06 remarks: filtered before SCX | LTQ | PMID:19260763 19260763 | |||||||
| PAe000088 | Yeast, TSAA time 90 experiment, cleavable ICAT | TSAA time 90 experiment cleavable ICAT T=90 timepoint measures proteome expression in synchronous cells 90 min after release from alpha-factor. 2 mg TCA-precipitated protein from budding yeast cells synchronized using the mating pheromone alpha-factor were heavy-cICAT labeled, and 2 mg aynchronous baseline sample were light-cICAT labeled, 3 hrs, 37�C. Pre-labeling reduction in 5 mM TCEP, 60 min 37�C. Trypsinization in 15 ml (20 mM Tris pH 8.3, 5 mM EDTA, 12 ng/�l trypsin) 37�C overnight. Strong cation exchange on Polysulfoethyl A (PolyLC 200 mm x 4.6 mm), dogleg 800 method, 0.8 ml/min, 1 min fractions. 30 peak fractions manually avidin-purified, acid cleaved as per ABI protocol. | LTQ | PMID:17133367 | |||||||
| PAe000086 | Yeast, TSAA time 30, cleavable ICAT | TSAA time 30 experiment cleavable ICAT T=30 timepoint measures proteome expression in synchronous cells 30 min after release from alpha-factor. 2 mg TCA-precipitated protein from budding yeast cells synchronized using the mating pheromone alpha-factor were heavy-cICAT labeled, and 2 mg aynchronous baseline sample were light-cICAT labeled, 3 hrs, 37�C. Pre-labeling reduction in 5 mM TCEP, 60 min 37�C. Trypsinization in 15 ml (20 mM Tris pH 8.3, 5 mM EDTA, 12 ng/�l trypsin) 37�C overnight. Strong cation exchange on Polysulfoethyl A (PolyLC 200 mm x 4.6 mm), dogleg 800 method, 0.8 ml/min, 1 min fractions. 30 peak fractions manually avidin-purified, acid cleaved as per ABI protocol. | LTQ | PMID:17133367 | |||||||
| PAe000089 | Yeast, TSAA time 120 experiment, cleavable ICAT | TSAA time 120 experiment cleavable ICAT T=120 timepoint measures proteome expression in synchronous cells 120 min after release from alpha-factor. 2 mg TCA-precipitated protein from budding yeast cells synchronized using the mating pheromone alpha-factor were heavy-cICAT labeled, and 2 mg aynchronous baseline sample were light-cICAT labeled, 3 hrs, 37�C. Pre-labeling reduction in 5 mM TCEP, 60 min 37�C. Trypsinization in 15 ml (20 mM Tris pH 8.3, 5 mM EDTA, 12 ng/�l trypsin) 37�C overnight. Strong cation exchange on Polysulfoethyl A (PolyLC 200 mm x 4.6 mm), dogleg 800 method, 0.8 ml/min, 1 min fractions. 30 peak fractions manually avidin-purified, acid cleaved as per ABI protocol. | LTQ | PMID:17133367 | |||||||
| PAe000124 | Yeast, TSAA Time 0 Experiment | Yeast test experiment. Time 0 step using original ICAT reagents. See new CL-ICAT experiments for better data. | LTQ | ||||||||
| PAe000324 | yeast_quality_control | yeast_quality_control Yeast cell lysate was run on LTQ, LTQ_FT and QTOF. | Yeast | BY4741 (MATa, leu2D0, met15D0, ura3D0, his3D1) | LTQ | Yeast grown in synthetic complete medium at 30°C to log phase, harvested and diluted into 30mL synthetic complete media to a cell count of 3 × 10⁶/mL. | A 150µL portion of reduced yeast protein lysate was alkylated in 25mM of iodoacetamide and diluted to 1M urea with 50mM ammonium bicarbonate. 12µg of Trypsin Gold (Promega, Madison, WI) was added to the reduced and alkylated yeast protein lysate and trypsin digest was carried out overnight at 37°C. Trypsin was inactivated by addition of glacial acetic acid and insoluble material was removed by centrifugation. Peptides were purified using 3 mL SPEC C18 columns and concentrated in a SpeedVac. | After 4 h of growth at 30°C cells were harvested and washed three times with ice cold dH₂O. Cells were lysed by incubation with 1mL of ice cold 10% TCA for 1 h at 4°C. Protein precipitates were collected by centrifugation, washed twice with 1 mL cold 90% acetone, and dried in a SpeedVac (Thermo Savant, Holbrook, NY). Proteins were solubilized in 300µL of 8 M urea, 50mM ammonium bicarbonate and reduced by incubation at 56°C for 1 h in the presence of 15 mM DTT. | Unfractionated. | PMID:16823959 | |
| PAe000328 | yeast_lisa3_cam | yeast_lisa3_cam yeast cell lysate | LTQ | ||||||||
| PAe000326 | yeast_lisa2_cam | yeast_lisa2_cam yeast cell lysate | LTQ | ||||||||
| PAe000327 | yeast_lisa1_cam | yeast_lisa1_cam yeast cell lysate | LTQ | ||||||||
| PAe001697 | WCL_Hs_Tcell_WholeCellLysate9 | WCL_Hs_Tcell_WholeCellLysate9 | LTQ | PMID:17519225 | |||||||
| PAe001757 | WCL_Hs_Tcell_WholeCellLysate8 | WCL_Hs_Tcell_WholeCellLysate8 | LTQ | PMID:17519225 | |||||||
| PAe001749 | WCL_Hs_Tcell_WholeCellLysate7 | WCL_Hs_Tcell_WholeCellLysate7 | LTQ | PMID:17519225 | |||||||
| PAe001725 | WCL_Hs_Tcell_WholeCellLysate6 | WCL_Hs_Tcell_WholeCellLysate6 | LTQ | PMID:17519225 | |||||||
| PAe001701 | WCL_Hs_Tcell_WholeCellLysate5 | WCL_Hs_Tcell_WholeCellLysate5 | LTQ | PMID:17519225 | |||||||
| PAe001730 | WCL_Hs_Tcell_WholeCellLysate4 | WCL_Hs_Tcell_WholeCellLysate4 | LTQ | PMID:17519225 | |||||||
| PAe001732 | WCL_Hs_Tcell_WholeCellLysate3 | WCL_Hs_Tcell_WholeCellLysate3 | LTQ | PMID:17519225 | |||||||
| PAe001740 | WCL_Hs_Tcell_WholeCellLysate2 | WCL_Hs_Tcell_WholeCellLysate2 | LTQ | PMID:17519225 | |||||||
| PAe001704 | WCL_Hs_Tcell_WholeCellLysate14 | WCL_Hs_Tcell_WholeCellLysate14 | LTQ | PMID:17519225 | |||||||
| PAe001707 | WCL_Hs_Tcell_WholeCellLysate13 | WCL_Hs_Tcell_WholeCellLysate13 | LTQ | PMID:17519225 | |||||||
| PAe001754 | WCL_Hs_Tcell_WholeCellLysate12 | WCL_Hs_Tcell_WholeCellLysate12 | LTQ | PMID:17519225 |
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